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Transient transfection of the PX459-sgRNA into the target cancer cell lines was performed using the Lipofectamine 3000 Transfection Reagent (Invitrogen) in 6-well plates (Corning Falcon) followed by puromycin selection (Gibco, 15 g/ml) for 3 days

IAV infection has also been shown to suppress glucose-6-phosphate dehydrogenase expression, leading to decreased Nrf2 expression, induction of oxidative stress, and viral replication 70

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Comparison of creatine ingestion and resistance training on energy expenditure and limb blood flow

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