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Along with resistance to treatment in some patients with glioblastoma, the inability to cross the bloodbrain barrier (BBB) is deemed another flaw of such methods of treatment [30]

References Demir S, Nawroth PP, Herzig S, Ekim Ustunel B

Epigenetic modifications affecting long-term gene expression DNA methylation changes: GHK-Cu may affect DNA methyltransferase activity altering cytosine methylation patterns

Firstly, basal media composed of Advanced RPMI (Gibco) and 1% GlutaMax (Gibco) was supplemented with CHIR99021 (Tocris) and 5ng/mL recombinant human noggin (Peprotech), was added to hiPSCs following the 24 h DMSO treatment previously described