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This method classifies variants into different groups based on variant annotations (pLOF, all missense, missense (1/5), missense (5/5)) and allele frequency thresholds (1%, 0.05%, 0.01%, 0.001%, and singletons) and applies four main testing strategies: BURDEN (classic burden test across allele frequency bins), SBAT (Set-Based Association Test assuming same effect direction), SKATO (Sequence Kernel Association Test Optimal for mixed effect directions), and ACATV (Aggregated Cauchy Association Test for Variants)

Launched in 2024, zone is part of their synthetic smokeless portfoliousing only premium nicotine produced in a lab rather than extracted from the tobacco plant

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The tax credits and how they are used to reduce tax liability was explained in the profile earlier