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The PPP has an oxidative phase (irreversible steps), which produces two NADPH, and a nonoxidative phase

Altered phosphorylation of cytoskeleton proteins in sickle red blood cells: the role of protein kinase C, Rac GTPases, and reactive oxygen species

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The test was done at the end of the experiment (after the 2 weeks of the treatment) and repeated three times for each rat, and the average value was computed