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The reaction incubated for 15 min and 6 l of 50 mM DTNB was added to each well

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Because substitution with glycine (G) may disrupt the FHA domain conformation, we additionally generated alanine (A) substitutions (K265A, K301A, E365A, and K265A/K301A)

Following overdoses, however, the GSH pool is depleted, leading to NAPQI binding of other proteins and protein damage