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The ligandin (non-substrate) binding site of human Pi class glutathione transferase is located in the electrophile binding site (H-site)

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Men with naturally high aromatase activity or those whose estrogen rises disproportionately to testosterone increases face greater risk

Regardless, once formed, a protein sulfenate would rapidly react with GSH to produce the mixed disulfide, and this could be the mechanism through which PSSG formed for some proteins in the cytosol during oxidative stress when H 2 O 2 is high enough to overcome a slow rate constant