Dendritic Cells and Fibroblasts within TLS Exclusively Expressed the Cystine/Glutamate Transporter, a Key Molecule in Glutathione Synthesis Pathway System x c is the cystine/glutamate transporter that transports extracellular cystine into cells, and its transport activity is regulated by the light chain subunit encoded by Slc7a11
Its primary action is inhibiting tyrosinase
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Immunofluorescence Cells grown on coverslips were fixed with cold methanol at 20 C and then incubated with the following primary antibodies: anti-LC3A/B rabbit monoclonal (1:100) (Cell Signaling Technology, Inc., New England Biolabs., UK), anti-Phospho-S6 Ribosomal Protein rabbit monoclonal (1:100) (Cell Signaling Technology), anti p53 mouse monoclonal (1:100) (Dako Cytomation, Glostrup, Denmark) and anti-Ki67 rabbit polyclonal (1:300) (Abcam, Cambridge Science Park, Cambridge, UK)