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Mass spec should show peak at expected molecular weight confirming identity

Based on these estimates, a single, optimized standard addition mixture was custom prepared so that the additions approximated integer multiples of the endogenous concentrations, thereby maximizing method accuracy

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A pathologist will then examine the skin sample under a microscope to check for the presence of autoreactive IgA antibodies, which are produced by the immune system when gluten-sensitive people ingest gluten