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The protein extraction methods were as described in the reference [29]

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(7) Tianqi Liu, Ying Liu, Xiaoyu Zhao, Liguo Zhang, Wei Wang, De Bai, Ya Liao, Zhenyuan Wang, Mi Wang, Jiaheng Zhang, Thermodynamically stable ionic liquid microemulsions pioneer pathways for topical delivery and peptide application, Bioactive Materials, Volume 32, 2024, Pages 502-513 (8) Malinda KM, Sidhu GS, Mani H, Banaudha K, Maheshwari RK, Goldstein AL, Kleinman HK