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Measure AMPK phosphorylation status in skeletal muscle or liver tissue to confirm MOTS-c activity this requires tissue biopsy and Western blot analysis

The microenvironment of the injured spinal cord is characterized by mitochondrial metabolic imbalances, leading to the excessive production of free radicals and the consequent peroxidation of cell membrane lipids, which results in spinal cord nerve cell damage and even death [3,4,5]

[7] Because serious glutathione depletion can occur under conditions of trauma and stress, maintenance of adequate glutathione levels may enhance immunocompetence and thus improve your ability to recover from illness or physical stress

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