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With respect to aerosol generation techniques, current machine-based aerosol generation techniques pose several challenges for assessing different product aerosols because many smoking machines and exposure systems were originally designed for use with combustible tobacco cigarettes and do not easily translate to the standard production of e-cigarette aerosols

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As the larval instar increases, mortality rate started decreasing for 1 CB and 2 CBs while for 3 CBs, almost 100% mortality was observed at 48 h of exposure for both 3rd and 4 th larval instars while, for 2 CBs, 8090% larval mortality occurred at 48 h of exposure

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