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Initially, only nausea and vomiting occurs, but unless treated within 72 h hepatic necrosis and failure develops

Transmission electron microscopy (TEM) Uterine tissue was cut into ~ 1 mm cubes and fixed by incubating in 2.0% paraformaldehyde, 2.5% glutaraldehyde (both electron microscopy grade), and 0.1 M Na-Cacodylate buffer (pH 7.4) overnight at 4 C

It is worth noting that, unlike for solid tumors in a primary or secondary site, -oxidation is required for metastasis-initiating cells that travel through circulation and colonize new organs 17

doi: 10.1053/j.gastro.2012.09.008 81 XiongXLKuangHAnsariSLiuTYGongJKWangSet al

An example week at the 2.5 mg initiation step: The documented protocol allows the injection day to be moved if needed as long as doses stay at least several days apart, but for research clarity a fixed weekday is cleanest