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(2007) Trends Pharmacol.Sci

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Preparation is Everything: Work on a clean, disinfected surface

Briefly, rat striatal tissues were homogenized in ice-cold lysis buffer (50 mM Tris, 150 mM NaCl, 2 mM EDTA, 1% NP-40, 0.5% sodium deoxycholate, pH = 7.4, with protease inhibitor (Sigma-Aldrich)), and rotated for 1 h at 4 C

Symptoms range from persistent fatigue, brain fog, and shortness of breath to more severe complications such as cardiovascular issues and multisystem inflammatory syndromes