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The guidelines noted that there are no prospective interventional studies comparing various crystalloid fluid volumes for initial resuscitation but reference observational studies and a retrospective study that demonstrated not administering 30 mL/kg of crystalloid fluids within three hours of sepsis identification was associated with higher mortality regardless of comorbidities such as end-stage renal disease and heart failure

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Serum sample: Detect directly II

Interestingly, recent studies have proposed that DNA methylation can lead to the exclusion of a histone variant (H2A.Z), preferentially located at active gene promoter 36,37

doi: 10.1016/j.kint.2016.04.008 26 ShingarevRGlezermanIG