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Berberine activates AMPK through a different mechanism than MOTS-c (via inhibition of complex I in mitochondria), so concurrent use may create additive rather than synergistic AMPK activationhigher risk of hypoglycemia without proportional metabolic benefit

Freezing creates ice crystals that can physically damage the peptide structure

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However, the potential benefits of intravenous vitamin C in cancer treatment remains controversial