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Second, an in vitro chromosome aberration assay was performed in Chinese Hamster Ovary (CHO) cells treated with AOD9604 (20, 50, 100, 200 g/mL) in the absence or presence of an S-9 metabolic activation system

They found that it wasnt possible to determine a clear lower limit for which any of these biomarkers can reliably indicate B12 deficiency, but that daily intakes above 4.0 g appear to have no additional benefit