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The heart tissue was minced and homogenized in the isolation medium in a ratio of 1:9, which contained (mmol/l): sucrose250, EDTA1, Tris-HCl25, pH 7.4

This section details the core enzymatic machinery and the multi-layered regulatory networks that govern glucoraphanin accumulation

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The glutathione concentration was calculated using the molar extinction coefficient ( = 13,600 mol 1 .cm 1 )