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For protein analysis, follicles were lysed using PRO-PREP Protein Extraction Solution (iNtRON), and protein quantification was carried out using the BCA Assay Kit (Sigma-Aldrich)
Certain metallic cations, such as copper (Cu) and iron (Fe 2+/3+ ) may contribute significantly to the generation of ROS
Another potent antioxidant, resveratrol, is a naturally occurring polyphenol found in grapes, berries, and red wine [216,217,218]
Note that for CJC-1295 With DAC, Bacteriostatic Water reconstitution is suitable for in vivo albumin-conjugation applications but must be confirmed free of thiol-containing impurities that could quench the reactive maleimide DAC linker