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Sterility is a major concernwithout proper cleanroom standards, bacterial contamination and endotoxins are very real possibilities," she says

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The tissue underwent graded dehydration by sequential immersion in 30%, 50%, 70%, 90%, 100%, and 100% ethanol, each for 1 h

Shmerling, MD, is associate professor of medicine at Harvard Medical School and Clinical Chief of Rheumatology at Beth Israel Deaconess Medical Center in Boston where he teaches in the Internal Medicine Residency Program

Once dried, the samples are stable at ambient conditions for extended periods, making DBS an attractive alternative to conventional venous blood collection