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To determine whether Albumax was a contributing factor for the difference in PS exposure and hemolysis observed between culture conditions, we incubated erythrocytes in Ringer solution +/- Albumax or in incomplete RPMI (iRPMI) +/- Albumax for 48 h at 4% hematocrit ( Figures 2C, D )

Fixed cells were analyzed with a ZeissLSM 980 fluorescence microscope equipped with an automated stage and the ZEN 3.1 (blue edition) software (Carl Zeiss Microscopy GmbH) or with NikonCSU Spinning Disk using a 40x air objective (NA = 0.95), hardware autofocus, and semi-automatic multi-position image acquisition: Random fields of view were selected based on DAPI signal, saved in the software, and imaged all at once

L., Niven, F

Huang, Yuzhao et al