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Musculoskeletal Disorders Current epidemiological evidence suggests that cruciferous vegetables play a key role in muscle and bone health, possibly due to the presence of glucosinolates and isothiocyanates

Extraction of nuclear and cytoplasmic fractions Cells were harvested and suspended in 200 L Buffer A (10 mM KCl, 0.1 mM EDTA, 10 mM HEPES, pH 7.9, 0.1 mM EGTA, 2 mM NaVO 3 , 5 mM -Glycerol phosphate, 0.15% NP-40, and 1 mM PMSF) and kept on ice for approximately 15 min

Impaired protein digestion may limit natural glutathione production

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Following these procedures guarantees data reproducibility across sessions