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Lifestyle factors such as poor diet, lack of exercise, and excessive alcohol consumption
It is likely that the larger number of statistically significant changes to the microbiome observed in the groups switched from WD to LD reflects their more numerous exposures to different diets, longitudinal changes in dietary exposure, the stress associated with diet switch, the negative energy state achieved by treatment and/or diet-driven weight loss, or a combination of these factors, but it may also be related to differences in terminal body weight and fat mass since both adiposity and diet composition have been shown to modulate the mouse gut microbiome profile
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In rodent knockout models where beta-3 adrenergic receptors were removed, the lipolytic response to AOD 9604 was eliminated, confirming the receptor-specific mechanism
