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Nuclei resuspended in this buffer show significantly reduced clumping as compared to those resuspended in PBS with 5% BSA
The protocol is simple and rapid with high yield
which is specifically tailored for tendons
the presence of SDS in the sample could interfere with downstream applications
Unlike a resin-based depletion kit (that only deals with microliter amounts of a sample)
MinuteTM Total Protein Extraction Kit for Insects Pack Size:50 Preps Nuclei resuspended in this bufferDespite significant variation in body organization, insects all have the same general body structure. They have segmented bodies divided into three regions: head, thorax and abdomen. The body segments are protected by a hard exoskeleton or cuticles. From a protein extraction point of view, the unique structure of an exoskeleton makes it very hard to homogenize. It is also very difficult to lyse cells protected by cuticle for total protein extraction.
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