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The PVDF membranes were incubated with primary antibodies overnight at 4 C, incubated with secondary antibodies at room temperature for 1 h, and visualized using the Bio-Rad ChemiDoc XRS + system

Reported roughly 28% to 30% average weight loss at higher doses, the highest reported in a large Phase 3 obesity trial

stained with 3,3-diaminobenzidine (DAB, K5007, DAKO, Denmark) counterstained with haematoxylin

Step 4: Depress the plunger slowly, allowing the water to run down the glass wall

In previous studies, the connection between effort-based motivated performance in humans and levels of various metabolites in the accumbal region, was measured using 1 H-MRS at 7 Tesla before performing a physical effort-based motivated task (MIFT) (16, 17)