Briefly, sodium bicarbonate, glucose, sodium pyruvate, and all 20 amino acids (except cystine and serine), biotin (B4639, Sigma), and vitamin B12 (V6629, Sigma) were added to the DMEM medium (#D9800-28, USBiological, Salem, MA) to match standard IMDM, supplement with 15% human AB serum and 15% dialyzed FBS (#SH300790340, Cytiva Hyclone), along with 10 ng/mL interleukin-3, 50 ng/mL stem cell factor and 2IU/mL erythropoietin
Arterioscler Thromb Vasc Biol 17 : 989995
AMPK and SIRT1: a long-standing partnership
The purified particles after drop-casting on glass were completely dried at room temperature, and then AFM micrographs were taken under frequency (300 kHz) in multimode SPM [27]
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