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Cells were washed with ice-cold PBS and lysed with a triple detergent lysis buffer supplemented with protease and phosphatase inhibitor cocktail

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Methods We conducted literature search of published studies in the English language focusing on oxidative stress and its association with recurrent pregnancy loss (RPL) utilizing the Medline and Cochrane databases from 2000 through January 2021

Importance of professional supervision Professional supervision is also a strong point where safety is concerned