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The impact of energy deprivation on the signaling pathways of erythrocytes was later disclosed in a mechanistic study: here, the authors demonstrated that removal of extracellular glucose and blockade of glycolysis by 2-deoxyglucose leads to fast depletion of cellular ATP

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Their identification largely depends on indirect evidence from genetic or biochemical studies, suggesting that key components of perception mechanisms remain undiscovered

NAD+ does play a background role by supplying energy for immune activity, but it does not drive detox the way glutathione does