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To investigate gene expression, qRT-PCR was performed using cDNA from lung tissue with Taqman pre-developed primerprobe sets (Applied Biosystems, University Park, IL, USA)

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Furthermore, it was reported that short-term hookah use (both tobacco-based and tobacco-free products) disrupts the autonomic nervous system regulation on the cardiac cycle, thereby causing a reduction in HRvariability, whichin turnmight aggravate the risk of coronary artery disease development [18]

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