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For cryo-EM analysis, peak fractions were pooled, mixed and concentrated to protein concentration of 10LmgLml 1

Tesamorelin (a GHRH analogue) and ipamorelin (a selective ghrelin receptor agonist) were designed to mimic the body's natural growth hormone secretion pattern when used together, but that pattern collapses if the dosing protocol is off by even 30 minutes

Histological verification of cannula placement was accomplished by the injection of 5 fast-green dye via the guide cannula following the completion of behavioral testing

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