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Labelled cells were then detached by trypsinization, pelleted, washed once with PBS, resuspended in 300 l ice-cold PBS and fixed by adding 700 l ice-cold absolute ethanol while gently vortexing

The so called (economic) region of the centre - located at the interfaces of the three federal states of Hesse, Lower Saxony and North Rhine-Westphalia - illustrates the complexity of intra-national borders as social and institutional constructs

You should report any symptoms including eye redness, light sensitivity, eye pain, small specks floating in your vision, or any changes in vision to your healthcare provider Increase in eye pressure within minutes of the injection