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Then, 100 l of substrate (tetramethylbenzidine) was added to each well after washing and incubated at room temperature for 30 min

Another example is CGA-N12, a synthetic antimicrobial peptide that exerts potent antifungal effects against Candida albicans by inducing reactive oxygen species (ROS) accumulation and collapsing the mitochondrial membrane potential, ultimately triggering apoptosis (Li et al., 2018)

To further evaluate the levels of joint inflammation and cartilage damage, histological analysis of rat ankle joint sections was performed

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The findings are summarized in Table 11