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Materials and Methods Fluorescent instrumentation Perkin-Elmer fluorescent spectrometer model 1000 set at excitation wavelength 475nm and its scale expansion gain control knob was adjusted so that a 10M solution of the bimane conjugate gave a full scale reading of 100 fluorescence units

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It is formed from three amino acids, viz

Hydroxocobalamin is the NHS-preferred B12 injection formulation, prescribed as a prescription-only medicine for confirmed deficiency states including pernicious anaemia and malabsorption disorders