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RT-qPCR analysis was performed on a QuantStudio 3 Real-Time PCR Detection System using ChamQ Universal SYBR qPCR Master Mix (Vazyme, Q711-02) with specific primers

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Acknowledgments Thank you to Mackenzie Langan for her assistance with the creation of challenge materials for this study, and to Shalini Ramachandra for her assistance with variable creation

35 There is conflicting evidence from low-quality trials on the effects of acupuncture on lumbar spinal stenosis