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Furthermore, they exhibited elevated levels of autoimmune antibodies in 17 out of 24 markers, including antibodies against the brain, hormone glands, joints, smooth muscles, cardiovascular tissue, and more

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The IgG variants were purified using CaptureSelect C H 1 columns (Thermo Fisher) prior to size exclusion chromatography (SEC) to isolate monomeric fractions using a Superdex 200 Increase 10/300 column (Cytiva Life Sciences) with an KTA Avant 25 (Cytiva Life Sciences)

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