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Indeed, immunoblot analysis revealed significantly decreased protein levels of FASN in livers from GSH-depleted mice (Figure 5, I and J), without decreased levels of Fasn mRNA (Figure 5K)

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Phospholipids and their hydroxy and hydroperoxy derivatives, including 15-HETE-PE and 15-HpETE-PE, were analyzed by LC/MS using a Dionex Ultimate 3000 HPLC system coupled on-line to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) using a normal phase column (Luna 3 m silica C18(2) 100 , 150 2.0 mm, Phenomenex)