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The sulfur atom of glutathione is close to the OH of Tyr9 in the active site (Grahn et al., 2006), but the hydroxyl group of Tyr9 has only a marginal effect on the ionization of glutathione as demonstrated by the small increase of its pK a value from 6.7 to 7.2 when Tyr9 was mutated into Phe (Pettersson and Mannervik, 2001)

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Osborn BH, Haney AF, Misukonis MA, Weinberg JB: Inducible nitric oxide synthase expression by peritoneal macrophages in endometriosis-associated infertility

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