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Several studies have shown that oxidative stress exacerbates ferroptosis through multiple signaling pathways, including System Xc-, Nrf2/KEAP1 pathway, and NOX4 (63)

These discoveries, based on proteomics and mass spectrometry, establish a foundational basis for exploring lactylation-modified proteins as biomarkers

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Genomic DNA regions of interest were immunoprecipitated using antibodies against BRD4 (Bethyl Laboratories, A301-985A50), H3K4me3 (Diagenode, C15410003), or GFP (Takara Bio, USA632592)