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Protein extraction and digestion for mass spectrometry For protein extraction and digestion, we used the in-strip protein digestion method, as previously described 21

ALT is considered a more specific and sensitive indicator of hepatocellular injury than AST

The cells were grown in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum, 100 U/ml penicillin and 100 g/ml streptomycin (GenDEPOT, Barker, TX, USA) at 37C in a humidified incubator with 5% CO2

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The stabilization of HIF1 enhances the expression of glucose transporter proteins and hexokinase