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Chromatographical separation of metabolites was performed on Syncronis HILIC UHPLC column (2.1 mm 100 mm 1.7 m, Thermo Fisher) at the flow rate of 600 L/min using a binary solvent system: solvent A, 20 mM ammonium formate, pH 3.0 in mass spectrometry grade H 2 0, and solvent B, mass spectrometry grade acetonitrile with 0.1% formic acid (%v/v)

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After 68 weeks, weight loss was higher with one-weekly subcutaneous semaglutide 2.4 mg compared with once-daily liraglutide 3.0 mg ( 15.8% vs

The absence of reported adverse effects in short-duration animal studies is not equivalent to confirmed long-term safety