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Lactate Dehydrogenase (LDH) Assay In this assay performed on HaCaT keratinocytes cells, the cells were treated with GHK, GHK-Cu, CuCl 2 and Cu(OAc) 2 for 30 mins at 7 different concentrations each, ranging from 0.0058 to 5800 M0.0058 M, 0.058 M, 0.58 M, 5.8 M, 58 M, 580 M, 5800 M

In contrast, our study confirmed decreased glutathione levels and increased ROS levels through BSO treatment or GCLC silencing, and revealed that while the depletion of glutathione did not compromise lymphoma cell survival in 2D culture, it sharply diminished viability in 3D gels

In our experiments with DMF, we showed that the NRF2-GSH axis could be potentially targeted independently of melatonin

What it does

2-D electrophoresis was performed to elucidate the reasons for this discrepancy, and the results indicated obvious changes in central metabolism and amino acid metabolism in the penta-mutant