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Compound Name: Glutathione (GSH)

It is used in the body to make hormones, vitamin D, and substances that help digest foods

For cell number measurements in 6-well plates, the medium was aspirated, the wells were rinsed with PBS and the cells were detached by incubating with 500 l of 0.25% trypsin (Thermo Scientific, 25200056) for 20 min at 37 C

Consistent with the result that Ser184 phosphorylation was essential for GSTP to enter nucleus, both immunoblotting and confocal microscopy detection demonstrated that GF109203X inhibited LPS-triggered GSTP nucleus translocation, and PMA promoted GSTP to enter the nucleus in LPS-untreated RAW264.7 cells (Figures 6F,G)

Nrf2 plays a crucial role in protecting cells from oxidative damage, inflammation, and toxins by activating the expression of protective genes