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Determination of macromolecular substances Sample pretreatment involves using a soft brush to clean the dust from the surface of the tobacco leaves, removing the main vein, cutting them into fine strands, drying them in a 60 oven, grinding them into powder, and passing them through a 60-mesh sieve for storage.The starch, cellulose and lignin, and protein contents were determined according to YC/T 2162013, YC/T 3472010, and YC/T 2492008, respectively 29,30

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By 1948, the health effects experienced by those exposed to EtO had been observed, and a study found that it potentially causes cancer (USEPA 2016b)