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Wick W, Dettmer S, Berberich A, Kessler T, Karapanagiotou-Schenkel I, Wick A, et al

UMN culture is an important tool to investigate UMN responses to treatment in vitro UCHL-eGFP is a reporter mouse line for corticospinal motor neurons (CSMN, a.k.a the UMNs in mice), in which CSMN express eGFP, and CSMN identity of eGFP+ neurons in the layer 5 of the motor cortex is confirmed by retrograde labeling, molecular marker expression and electrophysiological properties 31

However, all these methods demand thorough preparation before implementation, thus utilization of high-throughput omics tools facilitates a comprehensive and integrated exploration of nutrition

The slide images were captured by an inverted microscope and the average optical density (AOD) was measured using Image J 1.53t software

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