PTM aims to induce oxidative and nitrosative stress in cancer cells [8, 12], potentially leading to their death while stimulating the immune system [13, 14]
In 1 mM PAP and 1 mM PAP-CYS treated cells, the cell density was significantly decreased after the treatment, thus based on the limitations of the assay, we analyzed only GCLC protein expression in cells treated with 0.1 mM PAP and 0.1 mM PAP-CYS
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This copper-peptide complex exhibits distinctive blue coloration characteristic of copper coordination compounds and demonstrates enhanced biological activity compared to the peptide alone
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