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Epitope spreading of the anti-citrullinated protein antibody response occurs before disease onset and is associated with the disease course of early arthritis

After protein extraction, Filter-Aided Sample Preparation (FASP) was used for digestion

Using bacteriostatic water for peptides, commonly known as BAC water, helps reduce contamination during multi-dose vial usage

The plates were washed twice by submersion in tap water followed by inversion and shaking and were tapped against a paper towel after the second wash to remove excess liquid

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