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Flow cytometry was performed using a BD Fortessa instrument (BD) or NovoCyte Quanteon and data were analyzed using FlowJo v10.6.1 software (Tree Star)

Free Radic Res 49:453471 Gill SE, Parks WC (2008) Metalloproteinases and their inhibitors: regulators of wound healing

Aim for 1.2 to 1.6 grams per kilogram body weight daily

were supported by Program Grant APP1072113 from the Australian National Health & Medical Research Council (NHMRC) and NHMRC Principal Research Fellowships to G.F.K

As the major constituents of the BBB, endothelial cells connect via gap junctional proteins and works together with various other cell types, such as astrocytes, pericytes, microglia, NG2-glia, and oligodendrocytes