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GLRX activity values were compared using a one-way ANOVA with Dunnetts multiple comparisons, with each mutant compared to the activity of the WT protein

PMID 1731620

The concentration of ascorbic acid (AsA) was determined from extracted fresh sample in 6% (w/v) trichloro acetic acid (TCA) solution and the supernatant was recovered by centrifugation 14,000 g for 15 min, 4 C

These results showed that neither alcohol use nor older age is associated with a higher incidence of liver cancer in METH users (Table 2), indicating that METH use is an independent risk factor for liver cancer

The study selected 2.5 mM DTNB for further experiments