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Jobira et al.(2020) conducted a prospective case-control study demonstrating that obese adolescent PCOS patients exhibit reduced -diversity (Shannon diversity), significantly lower relative abundance of Bacteroidetes , and increased Actinobacteria and Streptococcaceae , all of which closely correlate with testosterone levels and insulin resistance ( Bacteroidaceae and depletion of Prevotellaceae were significantly associated with metabolic parameters

RadjabzadehDBoschJAUitterlindenAGZwindermanAHIkramMAvan MeursJBJet al

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It is therefore crucial to develop robust and widely adopted live cell assays that can independently measure the accumulation of small molecules across the OM, beyond just relying on MIC values