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Finally, the samples were prepared for Western blotting analysis by lysing the brain homogenates on ice for 300 minutes in 100 mL of a lysis buffer composed of 120 mM NaCL, 40 mM Tris (pH 8), and 0.1% NP40, followed by centrifugation

Since it is a modified fragment of human growth hormone that doesnt affect growth or insulin levels, it is safer than full human growth hormone therapy

J Gerontol A Biol Sci Med Sci 2017;72(6):733739

(2011) Facilitation of hippocampal synaptogenesis and spatial memory by C-terminal truncated Nle1-angiotensin IV analogs

This difference is likely to be related to the more tightly packed ECD interface of RAMP2 and CTR, driven by the interactions with P37 Cagri , and the preferential CTR engagement by Cagri via a bypass conformation, as discussed below in the context of CTR complexes