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The first patient was a 64-year-old man from Rio Grande do Sul, Brazil, who developed congestive heart failure with bilateral pulmonary infiltrates and pleural effusion on chest X-ray in June 2016

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Cell lines and culture conditions HepG2 cells, a human hepatoma cell line, were obtained from the American Type Culture Collection (Manassas, VA) and were cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% (v/v) fetal bovine serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere

The absence of named CEOs, active emails, phone numbers, and postal addresses obstructs work of governance institutions and citizen interaction